Fig. 4.

ASFV targets AKT. (a) Vero cells were either mock infected or infected with Ba71V (MOI 5) for 1 h before residual virus was washed off and the 0 h time point was harvested. The remaining infected cells were incubated in 2 % media and harvested at multiple time points over a 16 h time course of infection. Control cells were either non-starved (NS) in complete cell media, starved (ST) in EBSS, incubated in media containing DMSO or incubated in media containing 50 nM LY294002 for 3 h. (b) Vero cells were incubated with mock inoculum (mock infected) or Ba71V (MOI 5) for 1 h. Inocula were removed and cells were incubated for a total of 4 h. During the last 2 h, cells were incubated in regular 2 % media or in 2 % media containing 200 nM Torin1 or 5 µM MK-2206 or a combination of both drugs. Separately, cells were starved in EBSS in the presence of both drugs. (a, b) Cells were then lysed, processed for immunoblot and probed with antibodies against the indicated proteins. The positions of molecular mass markers are indicated to the left of the gels.