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. 2021 Oct 13;6(5):e00474-21. doi: 10.1128/mSphere.00474-21

TABLE 2.

Plasmids used in this study

Plasmid name Description Addgene no. GenBank no.
pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy) Used to generate Spy.dCas9 cells (strain i1) 171089
pSAG1-mNeonGreen_TUB1-dTomato Used to generate dual-fluorescence reporter cells (strain i2) MZ090944
pTUB1-Sth.dCas9-P2A-CAT-T2A-TagBFP Used to generate Sth.dCas9 cells (strain i3) 171090
pU6-Universal Recipient plasmid for cloning S. pyogenes spacers via BsaI restriction sites (2) 52694
pU6-Universal.Sth.dCas9 Recipient plasmid for cloning S. thermophilus spacers via BsaI restriction sites 171088