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Experimental protocol. Mice were divided into 4 cohorts: (A) Experiment I, determination of the optimal ischemia duration to induce transient ischemic attack (TIA). (B) Experiment II, comparation of the methods for cerebral blood flow (CBF) monitoring. (C) Experiment III, investigation of the histopathological changes induced by TIA. (D) Experiment IV, exploration of the therapeutic effects of Dl-3-N-butylphthalide (NBP) on TIA.
