Fig. 2. Targeting Voltair^IM to membranes of specific endocytic organelles:

(a) Schematic of targeting strategy: Voltair^IM undergoes scavenger receptor mediated endocytosis by binding scavenger receptors. Endocytosed Voltair^IM traffics in a time-dependent manner from the plasma membrane to the early endosome, the late endosome and then the lysosome. (b) Representative colocalization between internalized Voltair^IM (red channel) and human macrophage scavenger receptors (hMSR1-CFP, green channel) transfected in HEK 293T cells. (c) Normalized intensity of Voltair^IM uptake in cells, in absence and presence of mBSA (30 eq.) Error bar represents mean ± s.e.m. of three independent trials, n =30 cells (d) Representative colocalization between various endocytic organelle markers (green channel) and Voltair^IM (red channel) at the indicated chase times. Early endosomes are labelled with Transferrin-Alexa546 (Tf), late endosomes are labelled with Rab7-mRFP (Rab7) and lysosomes are labelled with TMR dextran (Dextrans). Scale bar = 5 μm. (e) Pearson’s correlation coefficient (PCC) of Voltair colocalization (CL) with corresponding endocytic markers in (d) along with pixel shift (PS). Error bar represents mean ± s.e.m. of three independent trials, n = 20 cells.