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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Nature. 2021 Jun 30;595(7868):606–610. doi: 10.1038/s41586-021-03686-x

Fig. 1: fcAMP binding to intact HCN channels in ZMWs.

Fig. 1:

(a) Representative scanning electron microscopy (SEM) image of ZMWs. All ZMW chips used (n = 7) were confirmed by SEM. (b) Cartoon (not to scale) of ZMW with deposited eGFP-tagged HCN for fcAMP binding experiments (eGFP: PDB 2Y0G, HCN1: PDB 6UQG). (c-f) Representative fluorescence/time trajectories of fcAMP binding to HCN1SM and HCN2SM at 250nM and 750nM fcAMP overlaid with idealized fit (black). Only 120 of 300 seconds are shown. Additional trajectories provided in Extended Data Fig. 3-4.