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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Nature. 2021 Jun 30;595(7868):606–610. doi: 10.1038/s41586-021-03686-x

Extended Data Fig. 4: fcAMP binding to HCN2SM in ZMWs.

Extended Data Fig. 4:

Representative fluorescence trajectories of fcAMP (100 nM to 1500 nM) binding to HCN2SM in ZMWs with idealized fits (black) imaged at 100-ms resolution. The first 50 frames (grey) were removed from analysis.