Western-immunoblotted proteins treated with periodate followed by development with MAbs. (A) N. caninum 65-kDa protein; (B) B. caballi native RAP-1; (C) B. equi EMA-1/EMA-2 proteins. Lane 1, no treatment; lanes 2, 3, and 4, treatment with 0.1, 1.0, and 10 mM periodate, respectively. Numbers on the left are in kilodaltons.