Fig. 2. Measurement of methyl 1H relaxation and analysis of mobility for the FLN5+67 RNC. (A) 1H R2 measurements for the representative residue L661δ2 in isolated FLN5 and FLN5+67 RNC (298 K, 950 MHz). Measurements for other residues are plotted in Fig. S5.† (B) Distribution of R2 measurements in isolated FLN5, FLN5+67 RNC and the bL12 C-terminal domain (298 K, 950 MHz). (C) Illustration of the cancellation of Saxis2 terms through the comparison of dynamics in isolated FLN5 and FLN5 RNCs. (D) Correlation between 1H R2 rates and Saxis2τc values measured for L661δ2 in isolated FLN5 at varying concentrations of d8-glycerol, also illustrating the conversion from 1H R2 rates to Saxis2τc values for the FLN5+67 RNC. (E) Correlation between Saxis2τc values measured for methyl groups in isolated FLN5, and Saxis2τc values determined from 1H R2 rates for the FLN5+67 RNC. Error bars show standard errors propagated from R2 or cross-correlated relaxation measurements.
