FIG. 9.

Titration of m-AMSA in wild-type and recombination-deficient mutant infection. E. coli cells harboring the plasmid with both the ori and topo site were infected with either K10 or K10-46/uvsX in the presence of increasing concentrations of m-AMSA (0, 1, 2, 3, 5, and 10 μg/ml in lanes 1 through 6, respectively). DNA was purified from the infected cells, digested with AseI and HaeIII, and subjected to agarose gel electrophoresis. The resulting fragments were visualized by Southern hybridization with pBR322 as the probe.