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. 2021 Oct 13;7(42):eabg6467. doi: 10.1126/sciadv.abg6467

Fig. 7. Targeting epithelial tension during HRAS transformation.

Fig. 7.

(A) Schematic of the action of inhibitors. (B to E) Representative phase contrast images of confined nontransformed (Control) and HRAS-transformed MCF10A epithelia treated with ROCK inhibitor Y27632 (10 μΜ) (B and D) or myosin II inhibitor blebbistatin (25 μΜ) (C and E) during early transformation (6 hours) (B and C) or late transformation (24 hours) (D and E) Scale bars, 100 μm. (F and H) Time evolution of normal components of the traction-force field in the outer epithelial subdomains during early (F) and late (H) transformation in the presence of inhibitors. (G and I) Time evolution of the surface area of the epithelia’s footprint on the substate matrix during early (G) and late (I) transformation in the presence of inhibitors. Means ± SEM of medians at each time point (n = 3 to 5).