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Features of plasmid libraries used for multicopy suppression profiling
| MoBY-ORF library | Yeast tiling collection | Galactose inducible libraries | Random genomic fragment libraries | |
|---|---|---|---|---|
| PCR free | ✓ | ✓ | ||
| Promoter type | Native | Native | Inducible | Native |
| Plasmid copy number | Low | High | High | High |
| Barcoded | ✓ | |||
| Percent genome coverage | 90% | 97.2% | 80% | ~85% |
| Average fragment size | 2 kb | 10 kb | 1.5 kb | 5 kb |
| Number of genes per fragment | 1 | 4–6 | 1 | 2–3 |
| References | 69 | 66 | 67, 68 | 48 |
