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mNGS and PCR validation. mNGS in CSF sample from patient 1 revealed 34 unique reads of SHV1 with 2.43% coverage (A). mNGS in CSF sample from patient 2 found 29 unique reads of SHV1 with 2.55% coverage (B). PCR amplification with a 94 bp product of SHV1 further validated the results of mNGS (C)
