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. 2021 Sep 15;598(7880):368–372. doi: 10.1038/s41586-021-03906-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 8 — a, Analytical size-exclusion chromatography of EC* (left) and EC^TCR (right) in the presence of RTF1. Peak fractions were analysed by SDS PAGE. While RTF1 elutes with EC* in stoichiometric amounts, it elutes with EC^TCR sub-stoichiometrically, which is indicative of weaker association of RTF1 with EC^TCR compared to EC*. The experiment with EC*-RTF1 was performed once and with EC^TCR-RTF1 twice. For gel source data, see Supplementary Fig. 1. b, Modelling shows clashes between UVSSA and extended downstream DNA, and between SPT6 and extended upstream DNA, suggesting that some repositioning of DNA and/or SPT6 and UVSSA occurs when DNA is longer.