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. 2021 Oct 13;11:20371. doi: 10.1038/s41598-021-99923-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of subcultures on production of multiple shoots in TIB. Experiment carried out with 250 ml of culture medium and 6 numbers of inoculum used and 2-min immersion for every 6 h. results were recorded after 21 days of culture period. (Graph was generated using OriginLab Professional Version 2021b software; URL link: www.OriginLab.com/2021b).