. 2021 Oct 13;11:20371. doi: 10.1038/s41598-021-99923-4

Table 2.

Effect of the culture system, immersion frequency, and volume of culture medium on rooting, and shoot elongation.

Culture system	Rooting (%)	No. of roots/shoot	Root length (cm)	Shoot height (cm)
Semi solid culture	94.3 ± 1.5^c	5.65 ± 0.7^a	9.65 ± 1.0^d	09.25 ± 0.2^d
TIB-Immersion frequencies (2-min immersion)¹
4 h	97.6 ± 1.7^ab	6.0 ± 1.0^a	10.3 ± 1.0^cd	11.1 ± 0.6^c
6 h	98.6 ± 1.0^a	6.5 ± 0.7^a	12.0 ± 1.1^b	16.4 ± 0.9^a
12 h	96.1 ± 2.0^abc	6.3 ± 0.8^a	14.4 ± 0.8^a	17.8 ± 1.9^a
Volume of culture medium (ml)²
100	95.3 ± 2.7^bc	5.5 ± 0.9^a	12.7 ± 1.5^b	14.5 ± 1.8^b
250	98.6 ± 1.1^a	6.3 ± 0.4^a	11.5 ± 1.2^bc	17.5 ± 1.4^a
500	96.4 ± 2.2^abc	6.0 ± 1.1^a	12.8 ± 1.4^b	17.7 ± 1.7^a

Results were recorded after 84 days of culture duration. Numbers represent the mean ± SD. Means with different letter are significantly different (DMRT, p ≤ 0.05).

¹Experiment carried out with 250 ml of culture medium and 150 numbers of inoculum used.

²Experiment carried out with 2-min immersion for every 6 h and 150 numbers of inoculum used.