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. 2021 Oct 13;12(10):940. doi: 10.1038/s41419-021-04226-1

Fig. 4. PARK7 regulates the Nrf2 signaling pathway.

Fig. 4

The effect of PARK7 on the interaction between Nrf2 and Keap1. A Immunoprecipitation and immunoblot analysis of Nrf2-Keap1 interactions in BMSCs (n = 3). Keap1 Kelch-like echinacoside (ECH)–associated protein 1. B Quantification of immunoprecipitated Keap1 is shown in A (n = 3). C Quantification of immunoprecipitated Nrf2 is shown in A (n = 3). D Quantification of input Keap1 is shown in A (n = 3). E Quantification of input Nrf2 is shown in A (n = 3). PARK7 affects the entry of free Nrf2 into the nucleus. F Immunoblot analysis of N-Nrf2, C-Nrf2, and T-Nrf2 protein expression in BMSCs (n = 3). N-Nrf2 nuclear Nrf2, C-Nrf2 cytoplasmic Nrf2, T-Nrf2 total intracellular Nrf2. G Immunoblot analysis of the purity of cytosolic and nuclear proteins (n = 3). Histone 3 (H3) was used as reference nuclear protein and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) as reference cytoplasmic protein. H Quantification of N-Nrf2 expression is shown in F (n = 3). I Quantification of C-Nrf2 expression is shown in F (n = 3). J Quantification of T-Nrf2 expression is shown in F (n = 3). All data are represented as mean ± SD. *P < 0.05. Differences were tested using one-way ANOVA with Tukey’s post hoc test (B–E, H–J).