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. 2021 Oct 13;11:20316. doi: 10.1038/s41598-021-99953-y

Table 1.

Forward and reverse primer sequences used in the qRT-PCR.

Primer name Primer sequences
NF-кB Forward 5′-TGCTAATGGTGGACCGCAA-3′
Reverse: 5′-CACTGCTTCCCGAATGTCTGA-3′
iNOS Forward: 5′-ACCATGGAGCATCCCAAGTA-3′
Reverse: 5′-CAGCGCATACCACTTCAGC-3′
COX-2 Forward: 5′-CCCATGTCAAAACCGTGGTG-3′
Reverse: 5′-CTTGTCAGGAATCTCGGCGT-3′
TNF-α Forward:5′-GCCCAGACCCTCACACTC-3′
Reverse: 5′-CCACTCCAGCTGCTCCTCT-3′
COL1A1 Forward: 5′-CATGTTCAGCTTTGTGGACCT-3′
Reverse: 5′-GCAGCTGACTTCAGGGATGT-3′
TGF-β1 Forward: 5′-TGCTAATGGTGGACCGCAA-3′
Reverse: 5′-CACTGCTTCCCGAATGTCTGA-3′
IL-1β Forward: 5′-GGGCCTCAAGGGGAAGAAGAATC-3′
Reverse: 5′-ATGTCCCGACCATTGCTGTT-3′
IL-8 Forward: 5′-GAAGATAGATTGCACCGATG-3′
Reverse: 5′-CATAGCCTCTCACACACATTTC-3′
β-Actin Forward: 5′-AAGCAGGAGTATGACGAGTCCG-3′
Reverse: 5′-GCCTTCATACATCTCAAGTTGG-3′

NF-κB nuclear factor-kappa B, iNOS inducible nitric oxide synthase, COX-2 cyclooxygenase -2, TNF-α tumor necrosis factor-α, COL1A1 collagen type I alpha one chain, TGF-β1 transforming growth factor-β1, IL interleukin.