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. 2021 Oct 13;7:292. doi: 10.1038/s41420-021-00674-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A RT-PCR unveiled the CASC9 expression in human glioblastoma cell lines (U87MG, U251) relative to normal human astrocytes (NHA). B ShRNA-expressing lentiviruses targeting CASC9 were transfected into U87MG cells. CASC9 overexpression plasmids were transfected into U251 cells. RT-PCR unveiled the CASC9 expression relative to the control group. C Glucose uptake analysis, D lactate production and E ATP generation analysis were performed in U251 cells and U87MG cells to control group (vector or shRNA). F Extracellular acidification rate (ECAR) analysis was performed for the glycolysis stress test in U251 cells and U87MG cells. G Oxygen consumption rate (OCR) assay was performed for the respiratory rate in U251 cells and U87MG cells. Data are presented as the mean ± SD. **P < 0.01, *P < 0.05.