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. 2021 Oct 13;12:5970. doi: 10.1038/s41467-021-26233-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Top, aggregation plots of piRNA reads surrounding the 5′ and 3′ splice sites of uppl mRNAs from the adult mouse testes. Bottom, the signal was calculated for non-genome matching piRNA reads mapping to exon–exon junction sequences. b Boxplots showing spliced RNA transcript length distributions. Control mRNA n = 43, uppl mRNA n = 30. c Boxplots showing exon length distributions. Control mRNA first or last exon n = 43, uppl mRNA first or last exon n = 30. Control mRNA middle exon n = 145, uppl mRNA middle exon n = 69. d Boxplots showing ORF and UTR length distributions. Control mRNA n = 43, uppl mRNA n = 30. e Discrete Fourier transformation of the distance spectrum of 5′-ends of RPFs across ORFs (gold) and 3′UTRs (green) of uppl mRNAs in adult wild-type testes. f Boxplots showing translational efficiency (RPF abundancy divided by mRNA abundancy) in adult wild-type testes. Control mRNA n = 43, uppl mRNA n = 30. Box plots in (b–d and f) show the 25th and 75th percentiles, whiskers represent the 5th and 95th percentiles, and midlines show median values.