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. 2021 Oct 13;12:5970. doi: 10.1038/s41467-021-26233-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Sequence logos depicting nucleotide (nt) bias at 5′-ends and 1 nt upstream of 5′-ends. Top to bottom: RPF species, anti-HA-immunoprecipitated 5′P RNA species, and piRNAs in adult testes, which map to mRNAs, uppl mRNA ORFs, and 3′UTRs, respectively. b Boxplots of the ratios of 5′P RPF versus 5′OH RPFs in adult wild-type testes. Control mRNA n = 43, uppl mRNA n = 30. c Boxplots of the ratios of RPF abundance in monosome fractions versus those in polysome fractions in adult wild-type testes. Control mRNA n = 43, uppl mRNA n = 30. d Boxplots of the ratios of RNA abundance in CBP80 IP versus eIF4E IP in adult wild-type (upper) and Mov10l1 CKO mutant (lower) testes. Control mRNA n = 43, uppl mRNA n = 30. e Aggregated data for RPF abundance from untreated adult testes (top) and from harringtonine-treated adult testes (bottom) across 5′UTRs, ORFs, and 3′UTRs of the uppl mRNAs (left) and control mRNAs (right). The x-axis shows the median length of these regions, and the y-axis represents the 10% trimmed mean of relative abundance. f piRNA abundance on uppl mRNAs (10% trimmed mean) in adult testes. Control (left) and Tdrd5 CKO (right). Box plots in (b–d) show the 25th and 75th percentiles, whiskers represent the 5th and 95th percentiles, and midlines show median values.