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. 2021 Oct 13;12(10):942. doi: 10.1038/s41419-021-04249-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — A HE and Masson’s trichrome staining of kidney tissue in the sham operation and UUO mouse models. Scale bar = 100 μm. B Representative bands of western blots showing the expression of fibronectin, collagen I, and α-SMA in the kidneys of wild-type and kidney-obstructed UUO mice. C–E Quantitation of fibronectin, collagen I, and α-SMA protein expression in B. *p < 0.05, **p < 0.01 vs. wild-type sham groups. F Immunohistochemical staining of GRP78 and S100A16 in the Sham operation and UUO mice models. Scale bar = 100 μm. G The expressions of GRP78, S100A16, and α-SMA were determined by western blotting of proteins from UUO mouse kidneys at 3, 7, and 14 days after UUO and comparing them with proteins from a control group. H–I Quantitation of GRP78 and S100A16 expressions in G, normalized to GAPDH expression.