Fig. 5. Ca2+ was required for ER stress-induced by S100A16 in HK-2 cells.
A Immunoblots showing the expression of renal fibrosis and ER stress-related genes in Lenti-scramble and S100A16-overexpressing HK-2 cells pretreated with BAPTA-AM (10 μM). B–G Quantitation of fibronectin, collagen I, α-SMA, GRP78, p-IRE1α/IRE1α, and XBP1s protein expression in A, normalized to GAPDH expression. Bars are means ± SE from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. H The cellular colocalization of S100A16 and GRP78. S100A16 in green, GRP78 in red, and ER tracker in blue. Scale bar = 20 µm.