Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 13;12:5969. doi: 10.1038/s41467-021-26183-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a In vitro acetylation assay of PopP2 variants in the presence of InsP6 and AcCoA, with RRS1_WRKY as substrate. This experiment was performed twice with similar results. The protein amount is indicated by Coomassie blue staining (bottom). b Electrophoretic mobility shift assay of PopP2 variants in the presence of InsP6, AcCoA, and W-box DNA, with RRS1_WRKY as substrate. Only unacetylated RRS1_WRKY proteins form complex with W-box DNA. The protein–DNA complexes and free DNA were detected by ethidium bromide (top), and the protein amount is indicated by Coomassie blue staining (bottom). c Immuno-detection of acetylated RRS1-R C-terminal portion in planta in the presence of WT PopP2 and PopP2-R380A mutant. Transient expression of 3HA-tagged PopP2 variants and eGFP-tagged RRS1-R_Cterm were performed in N. benthamiana leaves, with samples harvested at 48 hpi. Detection of HA- and eGFP-tagged proteins was conducted using anti-HA and anti-GFP antibodies, respectively. Ponceau S staining of total proteins indicates equal loading of the samples. This experiment was conducted three times with similar results. d Binding analysis results of WT and PopP2 variants. The K_d represents the mean value of two independent experiments.