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. 2021 Oct 13;4:1196. doi: 10.1038/s42003-021-02728-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Equal amounts of overexpression plasmids carrying ACE2 sequences from different species were transfected into 293 T cells. Transfection efficiency was confirmed by flow cytometry analysis of ACE2 surface expression. Cells were then infected with the same copy number of SARS-CoV-2 variants after quantification. Chemiluminescence signals were collected 24 h later. Ratios between variants and the D614G reference strain were calculated. The results were obtained from four independent experiments and the values shown indicate means ± SEMs. a human; b mouse; c pangolin; d bat; e mink; f ferret; g pig; h civet; i cattle; j sheep; k dog; l cat; m rabbit; n monkey. Dashed/dotted lines indicate the threshold of fourfold/twofold difference. Asterisks indicate statistical significance.