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. 2021 Oct 13;11:20293. doi: 10.1038/s41598-021-99207-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Localisation of clCRY2a/b in the pigeon retina. (a–f) Immunohistochemistry using the monoclonal clCRY2a/b antibody on adult pigeon retina harvested at midday (n = 3 birds). (a) Permanent staining reveals clCRY2a/b is localised throughout the retina and is enriched in the outer limiting membrane (OLM). (b) No staining is seen when 2A3 α clCRY2a/b is pre-incubated with the antigen. (c) Pigeon retinal sections treated with no primary clCRY2a/b antibody do not show any signal. (d–f) Immunofluorescence shows clCRY2a/b is enriched in the OLM and the outer plexiform layer (OPL). PR: photoreceptors, OLM: outer limiting membrane, ONL: outer nuclear layer, OPL: outer plexiform layer, INL: inner nuclear layer, IPL: inner plexiform layer, GCL: ganglion cell layer. Scale bars show 50 μm.