FIGURE 8.

SCH530348 suppressed IR-induced activation of PIK3 and NF-κB signaling. The protein levels of (A) IκB, (B) NF-κB, (C) Akt and (D) PI3K were detected by western blotting. Lamin B1 and β-actin served as loading controls for nuclear and cytoplasmic proteins, respectively. The protein levels of NF-κB, Akt and PI3K in lung tissue significantly increased and the protein level of IκB significantly decreased following exposure to IR. In addition, SCH530348 significantly attenuated the nuclear translocation of NF-κB, the degradation of IκB, and Akt and PI3K protein expression in the context of IR injury. The data are expressed as the mean ± SD (n = 4 per group). **p < 0.01, ***p < 0.001, compared with the control group; + p < 0.05, +++ p < 0.001, compared with the IR group.