FIG. 3.

dsRNA-induced inhibition of protein synthesis in RNase L^+/+ PKR^+/+, RNase L^−/− PKR^+/+, and RNase L^−/− PKR^−/− cells. The cells were grown in 12-well plates to ∼100% confluence in normal growth medium and then serum deprived for 24 hours in serum-free DMEM. Lipofectin (LF) mixes were made in leucine- and serum-free DMEM to contain 10 μg of pI · pC per ml and were given to the cells. Between 2.5 and 3 h after the pI · pC addition, the cells were pulse-labeled with 1 μCi of [³H]leucine per ml and processed further as described in Materials and Methods. Error bars indicate standard deviation from experimental points in triplicate determinations.