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. 2021 Sep 30;12:713795. doi: 10.3389/fpls.2021.713795

FIGURE 6.

FIGURE 6

Proposed Gal biosynthesis pathway analysis in the different MJ treated L. longituba seedlings. Phenylalanine (A), trans-cinnamic acid (B), 4-hydroxycinnamic acid (C), 3,4-dihydroxycinnamic acid (D), 4-hydroxybenzaldehyde (E), 3,4-dihydroxybenzaldehyde (F), tyrosine (G), tyramine (H), norbelladine (I), 4′-O-methylnorbelladine (J), N-demethylgalanthamine (K), and galanthamine (L) contents as measured by LC-MS/MS. Error values indicate the standard deviation (SD). Least-significant difference test (LSD, p < 0.05) was used to compare the means. Different letters represent significant differences between groups (N = 3, p < 0.05). (M) Proposed Gal biosynthesis pathway in L. longituba. Red or blue colors represent up-regulated genes or metabolites, green colors indicate down-regulated genes or metabolites, black colors represent non-detected metabolites or genes, and gray colors are genes or metabolites that did not change significantly under MJ-75 treatment. Heat maps showed the normalized gene expression values which represent the means ± SD of three biological replicates. Metabolites contents showed the means ± SD by LC-MS/MS. Gene expression values are presented as FPKM normalized log2-transformed counts (N = 3, p < 0.05).