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. 2021 Sep 30;12:713795. doi: 10.3389/fpls.2021.713795

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Copyright © 2021 Li, Xu, Zheng, Zhang and Cai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression analysis of the DEGs involved in Gal biosynthesis in the different MJ treated L. longituba seedlings. RT-qPCR analysis of PAL (A), C4H (B), C3H (C), OMT (D), CYP96T1 (E), TYDC (F), and NBS (G). The bar chart shows the relative expression levels as measured by RT-qPCR values, while the top vertical axis shows the relative RPKM values. Error values indicate the standard deviation (SD). C3H, p-coumarate 3-hydroxylase; C4H, Cinnamate-4-hydroxylase; CYP96T1, noroxomaritidine synthase; DEGs, differentially expressed genes; NBS, norbelladine synthase; OMT, norbelladine 4′-O-methyltransferase; PAL, phenylalanine ammonia; RPKM, Reads Per Kilobase per Million mapped reads; RT-qPCR, real-time quantitative polymerase chain reaction; TYDC, tyrosine decarboxylase. Least-significant difference test (LSD, p < 0.05) was used to compare the means. Different letters represent significant differences between groups (N = 3, p < 0.05).