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. 2021 Sep 30;12:726707. doi: 10.3389/fphar.2021.726707

FIGURE 5.

FIGURE 5

Antibiotics abolish the anti-inflammatory effects of metformin. Flow chart of animal treatment (A), weight loss and gain (n = 6–10) (B), disease activity index (DAI) scores (n = 6–10) (C), representative pictures of the mouse colon (n = 6–10) (D), colon length (n = 6–10) (E), hematoxylin and eosin (HE) staining (scale bars, 400 μm) (F), histological scores (n = 5–8) (G) *p < 0.05 (DSS+Met vs abx+DSS) and statistical comparison was made using an ANOVA test followed by Dunnett’s post-hoc tests. **p < 0.01, ***p < 0.00, # p < 0.05 (DSS+Met vs abx+DSS+Met), ## p < 0.01.