Figure 6.

Effects of pepD2M and pepD3M on the plant responses after the infection with Pectobacterium carotovorum subsp. carotovorum (Pcc). The Pcc cell suspension (10⁶CFU/ml in 10mM MgSO₄, 0.01% Silwet L-77) was mixed with the indicated peptide (64μg/ml) at a 3:1 ratio to produce a final peptide concentration of 16μg/ml. Crude peptides that were not purified via HPLC were used. Detached leaves of 4-week-old tomato L390 (A,B) plants and leaves of the intact Arabidopsis Col0 plants (C,D) were wounded with a 10μl micropipette tip and then droplet inoculated with 10μl of the Pcc-peptide mixture on the wounding sites. The disease symptoms were photographed 16–28h post-inoculation (A,C), after which the diameters of the lesions were measured (B,D). The data are from a single experiment that was repeated at least three times with similar results. (B,D) The values are the average±standard error (SE; n≥30). Pair-wise comparisons of the water- and peptide-treated samples were made using the Student’s t-test (^*** p<0.001; ^**** p<0.0001).