Figure 7.

Effects of pepD2M and pepD3M on the plant responses after the infection with Botrytis cinerea (Bc). The Bc spore suspension (10⁴ spores/ml for the tomato inoculation and 10⁵ spores/ml for the Arabidopsis inoculation) was mixed with the indicated peptide (64μg/ml) at a 3:1 ratio to produce a final peptide concentration of 16μg/ml. Crude peptides that were not purified via HPLC were used. Detached leaves of 4-week-old tomato L390 plants (A,B) and leaves of the intact Arabidopsis Col0 plants (C,D) were wounded with a 10μl micropipette tip and then droplet inoculated with 10μl of the Bc-peptide mixture. The disease symptoms were photographed 47–70h post-inoculation (A,C), after which the diameters of the lesions were measured (B,D). The data are from a single experiment that was repeated at least three times with similar results. (B,D) The values are the average±SE (n≥30). Pair-wise comparisons of the water- and peptide-treated samples were made using the Student’s t-test (^**** p<0.0001).