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. 2021 Sep 30;11:670353. doi: 10.3389/fonc.2021.670353

Figure 1.

Figure 1

Expression of NUDT21 in human gastric cancer/normal gastric tissue samples and cell lines. (A, B) Protein levels of NUDT21 in 70 gastric cancer tissues and 70 normal gastric tissues were examined by immunohistochemistry. The magnifications were 200 and 100 respectively. Red arrows: normal gastric tissue; black arrows: gastric cancer tissue. (C) Relative mRNA levels of NUDT21 in gastric cancer cells AGS, BGC-823, SGC-7901, HGC-27, MKN-28 and normal gastric cell GES-1 were examined by RT-qPCR. GAPDH was used as control. (D) Protein levels of NUDT21 in gastric cancer cells AGS, BGC-823, SGC-7901, HGC-27, MKN-28 and normal gastric cell GES-1 were examined by western blot. β-Actin was used as control. (E) mRNA levels of NUDT21 in 70 gastric cancer tissues and 70 normal gastric tissues were examined by RT-qPCR. (F) mRNA levels of NUDT21 in 31 gastric cancer tissues from patients with distant metastasis and 39 gastric cancer tissues from patients without distant metastasis were analyzed by RT-qPCR. (G) Kaplan-Meier curves showed the overall survival (OS) rates in gastric cancer patients with high and low levels of NUDT21. *P < 0.05; **P < 0.01.