Figure 6.

Defining transcriptional modules that distinguish SST+ interneurons born in vitro versus in vivo

(A) GO analysis of differentially expressed genes (unique genes with an average log2 fold change above 0.5) across SST+ interneurons from adult, fetal, and in vitro samples. In vitro samples consist of SST+ interneurons from iINs, mINs, and orgINs. Dot color represents the log10 p value for each term and the size represents the number of genes present per GO term.

(B) Heatmap of normalized counts in individual cells of the top differentially expressed genes between SST+ adult, fetal, and in vitro interneurons from (A), clustered according to their functional properties as defined by GO terms.

(C) TF module analysis, comparing differential expression of TFs and chromatin regulators across adult, fetal, and in vitro-derived SST+ interneurons. Normalized expression data are plotted and optimal leaf hierarchical clustering was used to generate modules of highly correlating regulators.

(D) The normalized expression of indicated TFs/chromatin regulators from different modules in (C) is depicted in violin plots.