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. 2021 Sep 30;11:668661. doi: 10.3389/fonc.2021.668661

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Copyright © 2021 Abramov, Dru, Belykh, Park, Bardonova and Preul

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Bar plot showing a comparison of the mean (SD) intensity modalities in the fluorescein sodium redose, initial-dose, and single-dose groups. The top and bottom whisker marks indicate the SD. *P < 0.001. The mean (SD) values of brightness and contrast of images are 79.1 (17.2) and 30.5 (6.3), respectively, in the redose group; 73.1 (20.4) and 25.4 (4.9) in the initial-dose group; and 54.6 (20.0) and 22.2 (7.9) in the single-dose group. The values are reported in units of optical density defined by Fiji open-source analytic software. Brightness is the mean gray value, calculated by the sum of the gray values of all the pixels in the selection divided by the number of pixels. Contrast is the SD of gray values used to generate the mean. SD, standard deviation. Used with permission from Barrow Neurological Institute, Phoenix, Arizona.