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. 2021 Sep 30;12:749468. doi: 10.3389/fimmu.2021.749468

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Copyright © 2021 Antequera, Moneo, Carrero, Bartolome, Ferrer, Proctor and Carro

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Muscarinic M3 receptor levels in mice and human submandibular glands. (A, B) Western blot analysis showed reduced M3 receptor levels in submandibular glands from (A) 6- and (B) 12-month-old APP/PS1 compared with WT mice (n = 8-13, per group). (C) In submandibular glands from AD patients, M3 receptor levels were also decreased compared with healthy control subjects (n = 8-10, per group). Representative Western blots and histograms with their densitometric analysis are shown. Data are represented as mean ± SEM. Differences between groups were assessed using the Mann-Whitney test. *p < 0.05; ***p < 0.001.