Table 5.

The methods used for HEV diagnosis.

Test	Method	Recommendation	Comments
IgM anti-HEV	Enzyme-linked immunosorbent assay	Acute phase of infection	Different performance between assays lower sensitivity and sensibility in patients with immune disorders
	Immunochromatographic test		Concerning regarding diagnostic yield according to the genotype
IgG anti-HEV	Enzyme-linked immunosorbent assay immunochromatographic test	Seroprevalence studies chronic infection natural protection after acute infection serologic status post vaccination	Different performance between assays
HEV RNA	Real time polymerase chain reaction	Acute phase of infection confirm chronic phase of infection especially in immune-compromised subjects to evaluate anti-viral response organ or blood donors screening	Different performance between in house assays short-lasting viremia load present advantage/better accuracy when testing among subjects with immune conditions
HEV antigen	Enzyme immunoassay	Acute phase of infection	Relatively high accuracy 81% concordance with nucleic acid test HEV RNA