Figure 5.

Inhibition ability of ANM to GD2⁺ cells for chemotherapy and gene therapy in vitro

(A) MYCN gene silencing by ANM in GD2⁺ IMR32 cells. ANM-high indicates IMR32 cells incubated with 20 nM ANM, and ANM-low indicates IMR32 cells incubated with ANM. The difference compared with the blank is labeled. ∗p < 0.05. (B) Rates (%) of apoptotic cells detected by TUNEL staining The difference compared with the blank is labeled. ∗p < 0.05, ∗∗p < 0.01. The difference compared with the IMR32 cells with the same treatment is labeled ^&p < 0.05, ^&&p < 0.01. (C) Fluorescence microscopy imaging of ANM-induced apoptotic cells, detected by TUNEL staining. Apoptosis cells were stained in green. Scale bars: 50 μM. (D) MTS assays in vitro after the cells were treated with ANM or ACM. The difference compared with the ANM group at the same concentration is labeled. ∗p < 0.05, ∗∗p < 0.01.