Table 4.

Differences of immunological parameters between first and second sampling.

	Children < 6 years) (n = 21)			Children 6–15 years (n = 26)			Adult > 15 years (n = 69)
	First sample	Second sample	p-value§	First sample	Second sample	p-value§	First sample	Second sample	p-value§
Median (IQR) months	2.4 (1.9–2.8)	5.6 (4.5–5.9)		3.3 (2.6–3.8)	6.6 (5.9–7.5)		2.8 (2.1–3.6)	6.1 (5.4–7.3)
logPRNT	5 (4–6)	6 (5–6.5)	0.521	5 (4–6)	5 (3–5)	0.672	4 (3–4)	3 (2–4)	0.004
%CD4 activation (CD4+HLA-DR+CD38+)	0.6 (0.5–0.8)	0.6 (0.5–0.8)	0.533	0.6 (0.5–1.1)	0.6 (0.4–1)	0.855	0.8 (0.4–1.3)	0.9 (0.6–1.1)	0.885
%CD8 activation (CD8+HLA-DR+CD38+)	0.7 (0.5–1.4)	0.7 (0.5–1.1)	0.750	0.8 (0.5–1.2)	0.8 (0.6–1.3)	0.618	1.2 (0.9–1.9)	1.7 (0.9–2.7)	0.058
%B activated memory (CD19+CD10−CD21−CD27+)	2.8 (1.5–4.8)	4.2 (2.5–5.7)	0.079	3.3 (2.3–4.5)	3.3 (2.3–4.8)	0.592	7.1 (4.1–11.7)	9.1 (4.7–11.8)	0.126
%CD4 senescence (CD4+CD28–CD57+)	0.6 (0.4–3.1)	3 (1.7–4.8)	0.047	0.8 (0.5–2.7)	6.5 (3.1–10)	<0.0001	2.5 (0.9–8.8)	6.3 (3.2–9.6)	0.026
%CD8 senescence (CD8+CD28–CD57+)	3.9 (2.0–7.8)	4.7 (2.7–7.9)	0.418	8.3 (4– 13)	8.4 (6.2–13.5)	0.956	13.8 (11–21.5)	14.7 (10.5–22.4)	0.484
%B senescence (CD19+IgD−CD27−)	10.9 (6.1–15.6)	7.8 (2.4–10.7)	0.051	14.9 (9–16.2)	13.4 (10.2–17.1)	0.303	16 (12.5–20.1)	16.5 (11.8–21.8)	0.868
%CD4 exhaustion (CD4+PD-1+)	7.7 (3.3–16.2)	7.3 (4.4–13.7)	0.927	9.2 (5.2–15.4)	10 (4.2–15.8)	0.825	13 (8.3–21.9)	11.5 (7.3–18.6)	0.430
%CD8 exhaustion (CD8+PD-1+)	9.5 (5.9–12.3)	7.8 (4.7–12.4)	0.870	9.6 (6.2–16.5)	9.9 (6.7–15)	0.719	14.2 (9.5–22)	12.2 (7.2–19.5)	0.213
%T-regs (CD4+CD25+CD127-FoxP3+)	8.3 (4.7–10)	7.9 (6.1–10)	0.498	4.5 (2.9–7)	4.7 (2.4–7.1)	0.658	1.4 (0.7–3.4)	1.0 (0.5–1.5)	<0.0001
%B-regs (CD19+CD24hiCD38hi)	6.1 (4.6-8.8)	7.4 (5.3–8.9)	0.468	5 (4–6.9)	5.2 (4.3–5.9)	0.310	2.1 (1.2–3)	1.2 (0.6–1.9)	<0.0001

^§Wilcoxon signed-rank test.

Bold values refer to statistically significant p-values.