Fig. 3.

Short-term (10 days) intestinal Mttp deletion mitigates hepatic steatosis but not fibrosis. A: Schematic diagram of experimental design. B: Biochemical quantitation of hepatic lipid content (n = 11–13/genotype). C: Representative images of Sirius red-stained liver tissue (200×) and quantitation of fibrotic area (right panel, n = 11–13/genotype; both male and female mice). D: Representative images of hepatic F4/80 staining (400×) and quantitation of F4/80-stained area (right panel, n = 7–9/genotype; both male and female mice). E: Relative abundance of the most abundant TG species presented as peak area ratio, with DNL, SCD, and elongation indices calculated from all TG species shown on right (n = 4/genotype). F: mRNA expression of genes related to fibrosis, inflammation, and lipogenesis (n = 7–13/genotype). G: Expression of total and phosphorylated JNK and NF-κB proteins in control and Mttp-IKO liver tissue. Representative Western blot (left) and quantitation of relative protein levels are shown, with levels of GAPDH as a loading control. H: Serum ALT (left) and AST levels (n = 9/genotype). I: Serum FITC-dextran (FD4) levels 2 h after oral gavage (n = 6/genotype). For all panels, data are presented as mean ± SEM. ∗P < 0.05 and ∗∗P < 0.01.