Fig. 5.

Switching from HFFC to chow diet reverses steatosis but not inflammatory or fibrogenic signaling in male Mttp-IKO mice. Abbreviations for all panels: HFFC, mice fed HFFC for 14 weeks; HFFC → chow, mice fed HFFC for 10 weeks and then chow for 4 weeks; HFFC → IKO chow, mice fed HFFC for 10 weeks and then chow for 4 weeks concomitant with induction of intestinal Mttp deletion. A: Representative images of H&E-stained liver tissue (400×). B: Biochemical quantitation of hepatic lipid content, n = 5–10/group. C: Representative images of Sirius red-stained liver tissue (200×), with quantitation of fibrotic area (right panel, n = 8–10/group). D: Collagen content determined by hydroxyproline detection and normalized to protein content, n = 4–5/group. E: Representative images of F4/80 staining (400×), with quantitation of F4/80-stained area shown at right (n = 4–5/group). F: Relative abundance of the most abundant TG species presented as peak area ratio (n = 5/group). G: Indices of DNL, saturation (SCD), and elongation calculated from peak area of all TG species (n = 5/group). H: Relative abundance of hepatic ceramide (Cer) species, with total Cer peak area ratio shown on the far right (n = 5/group). I: mRNA expression of hepatic Cer synthase isoforms (n = 5/group). For all panels, ∗P< 0.05 and ∗∗P < 0.01.