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. 2021 Sep 16;54(3):314–320. doi: 10.1055/s-0041-1734573

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Association of Plastic Surgeons of India. This is an open access article published by Thieme under the terms of the Creative Commons Attribution-NonDerivative-NonCommercial-License, permitting copying and reproduction so long as the original work is given appropriate credit. Contents may not be used for commercial purposes, or adapted, remixed, transformed or built upon. (https://creativecommons.org/licenses/by-nc-nd/4.0/).

This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives License, which permits unrestricted reproduction and distribution, for non-commercial purposes only; and use and reproduction, but not distribution, of adapted material for non-commercial purposes only, provided the original work is properly cited.

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Fig. 2 (A-F). Tissue analysis technique. — Manual tracing for quantifying the full-thickness skin graft (FTSG) surface area and nonviable region ( A, B ). Schematic illustration of the cross-sectional view ( C ) and clinical photograph of posteroanterior view of the explanted graft (D). Cross-section of H&E stain explant showing the three distinct tissue layers ( E ) and after threshold analysis of the same image selecting the collagen layers sandwiched between the skin graft and muscle tissue ( F ).