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. 2021 Sep 30;6(40):26239–26250. doi: 10.1021/acsomega.1c03243

Table 2. Vmax, Kasp, KM, α, and α′ Derived from Figure 1 Data.

solution (w/w) Vmax (μM/s)a Kasp (×10–3/s)a KM (mM)b SSRc αd α′d
control 0.291 ± 0.002 1.01 ± 0.03 0.28 ± 0.01 0.00042    
30% PEG 8 K 0.367 ± 0.004 0.62 ± 0.02 0.59 ± 0.02 0.000069 1.63 ± 0.07 0.79 ± 0.01
30% PEG 400 0.202 ± 0.002 0.54 ± 0.02 0.38 ± 0.02 0.00036 1.9 ± 0.1 1.44 ± 0.02
a

Values of parameters ±standard deviation fitting data to eq 4.

b

Value of Km ± standard deviation fitted to eq 1. Fitting to either eq 1 or eq 4 did not significantly alter Vmax.

c

Sum of squares residues of the fit to eq 4. Number of observations = 18 for each solution.

d

Values ± error estimate. Error calculated by propagating standard deviation of fitted Kasp and Vmax, respectively.