Figure 2.

Recommended plate setup for flow cytometry. Schematic showing an example of a plate layout for experiments comparing efflux capabilities of different strains (left) or putative efflux inhibitors (right). For strain comparison, each plate contains an unstained and stained parental control and efflux-deficient strain to ensure that a measurable window is achieved (yellow), in addition to the strains to be analyzed indicated by Sx, highlighted in blue. For assessing putative efflux inhibitors, a control compound should be used for the same purpose (yellow), in addition to stained samples containing molecules being assessed, indicated by Cx and highlighted in blue.