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. 2021 Oct 12;153(12):e202112969. doi: 10.1085/jgp.202112969

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© 2021 Eldstrom et al.

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Figure S3. — HMR blocks ML277-enhanced Q* currents, and ML277 blocks the S338F mutant KCNQ1 channel. (A and B) Tail currents from channels made up of (A) E160R-Q (Q*) and (B) GFP transfected cells, under control conditions (blue), after 1 µM ML277 (red), after 10 µM HMR + ML277 (green), and after washout (cyan). Cells were held at −80 mV, pulsed to +60 mV for 4 s, and then pulsed to −40 mV for 0.75 s. (C) Representative whole-cell current traces from cells before (blue trace) and after exposure to 1 µM ML277 (red trace) for KCNQ1 channel containing the S338F mutation. (D) Paired peak tail current measurements before and after ML277 treatment, measured after a 4-s pulse to +60 mV. (E) Bar chart of peak tail currents measured in ML277 divided by control peak current measurement for S338F mutant. The mean was 0.42 ± 0.10 (n = 4).