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. 2021 Oct 13;21:495. doi: 10.1186/s12872-021-02307-9

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — NaHS reduced aortic CAS activity and its protein expression, Stat3 phosphorylation, TGF-β1 protein level and up-regulated elastin protein level in DN rats. Western blot method was used to examine the protein expressions of Stat3, CAS, TGF-β1 and elastin in DN rats after 8 weeks of NaHS (50 μmol/kg/day) treatment. β-actin or total Stat3 from the same blot was control for protein loading. CAS: cathepsin S. C: control. Results are from one representative experiment and relative to total Stat3 or β-actin expression. n = 6 ~ 8 rats (C) and n = 3 ~ 5 rats (A, B, D, E). Values represent the means ± SEM. *p < 0.05 compared to control rats. #p < 0.05 compared to DN rats