Table 3.
Primer sequences used for the analysis of 13 ovarian ageing-related genes
| Gene ID Gene name |
Primer sequence (5' → 3') | Product length (bp) | Annealing temperature (°C) |
|---|---|---|---|
| NM_001127233.1 (P53) | F: ATGAACCGCCGACCTATCCT | 264 |
60.76 59.13 |
| R: GCGGATCTTGAGGGTGAAATAC | |||
| NM_013671.3 (SOD2) | F: TCCCAGACCTGCCTTACGACT | 244 |
61.1 60.4 |
| R: CCCTTAGGGCTCAGGTTTGTC | |||
| NM_009810 (CASP3) | F: GTCTGACTGGAAAGCCGAAAC | 205 |
58.9 59.5 |
| R: GACTGGATGAACCACGACCC | |||
| NM_007527 (Bax) | F: GCCTTTTTGCTACAGGGTTTCAT | 151 |
61.9 59.9 |
| R: TATTGCTGTCCAGTTCATCTCCA | |||
| NM_009741.5 (Bcl2) | F: GCTACCGTCGTGACTTCGCA | 270 |
61.9 61.6 |
| R: CATCCCAGCCTCCGTTATCC | |||
| NM_019584.3 (Beclin1) |
F: GCAGCAGTTCAAAGAAGAGGTG R: TTTTGATGGAATAGGAGCCGC |
122 |
60.3 58.7 |
| NM_026160.4 (LC3b) |
F: CGTCCTGGACAAGACCAAGTTC R: GCAAGCGCCGTCTGATTATC |
80 |
61.12 59.77 |
| NM_001159589.1 (sirt1) |
F: CAGCATCTTGCCTGATTTGTAA R: TGGGGTATAGAACTTGGAATTAGTG |
268 |
57.29 58.1 |
| NM_011034.4 (PRDX I) | F: CCACGGAGATCATTGCTTTCA | 139 |
58.64 58.55 |
| R: GGCCCAATCCTCCTTCTTTC | |||
| NM_001313711.1 (PRDX IV) |
F: TGTTGCGGACCGAATCTCTG R: CAATAAGGTGCTGGCTTGGAGA |
178 |
60.39 60.62 |
| NM_001358444.1 (PRDX V) |
F: AAGAAGCAGGTTGGGAGTGTG R: AACAGCCAGGTGTAAATGCCC |
201 |
60.48 61.17 |
| NM_001303408.1 (PRDX VI) |
F: CACCACAGGAACTGGCAGAC R: TGGTGGCAGGGTAGAGGAAG |
317 |
61.47 60.41 |
| NM_007393.3 (β-actin) |
F: GTGACGTGACATCCGAAAGA R: GTAACAGTCCGCCTAGAAGCAC |
287 |
58.7 61.0 |