FIG. 6.

Cardiac-specific 2-cM deletion between Hsd17b1 and D11Mit199. (A) PCR analysis of the deletion products. P1 and P2, primers used to specifically amplify the full-length Hprt. PCR reactions on αMyHC-Cre serve as a control. N, negative control; P, positive control; H, heart; M, skeletal muscle; Li, liver; Lu, lung; S, spleen. (B) Southern analysis of the deletion products. C, control cell line that contains a deletion and a wild-type chromosome. The cardiac-specific deletion band is indicated by an asterisk. N, NheI; S, SfiI; solid triangle, loxP site.