Fig. 1.

FB and FH during acute dengue infection in AG129 mice. Five- to six-week-old AG129 mice were mock-infected (n=6) or subcutaneously infected (n=6, day 2 p.i.; n=7, day 4 p.i.) with 10⁴ p.f.u./mouse of DENV-2 strain D2Y98P-PP1. At the indicated time points mice were euthanized, blood, liver and kidney harvested. (a) Circulating viremia was assessed by plaque assay including in n=4 animals kept until ethical moribund stage (day 17–21 p.i.). (b) Serum was subjected to ELISA for quantitation of FB and FH protein levels; (c) RNA was extracted from liver and (d) kidney and subjected to RT-PCR for FB and FH mRNA. mRNA levels are expressed relative to GAPDH using the ΔCt method. *P<0.05, one-way ANOVA/Tukey’s test.