TABLE 1.
Tentative LoD of the bKIT Virus Finder COVID-19 assay in NPS or BAL negative matrices
| LoD | SARS-CoV-2 (TCID50/ml) |
CT (mean ± SD) |
||
|---|---|---|---|---|
| Reaction mix 1 | Reaction mix 2 | RP | ||
| Tentative, in NPS | 1 × 104 | 26.89 ± 0.15 | 27.14 ± 0.19 | 25.26 ± 0.05 |
| 1 × 103 | 30.45 ± 0.07 | 30.68 ± 0.15 | 25.28 ± 0.03 | |
| 1 × 102 | 33.33 ± 0.63 | 34.1 ± 0.16 | 25.2 ± 0.04 | |
| 10 | 38.18 ± 0.53 | 37.68 ± 1.01 | 25.09 ± 0.16 | |
| 1 | N/A | N/A | 25.18 ± 0.02 | |
| Tentative, in BAL | 1 × 104 | 27.74 ± 0.06 | 29.30 ± 2.02 | 29.91 ± 0.17 |
| 1 × 103 | 31.14 ± 0.47 | 33.33 ± 0.44 | 30.82 ± 0.19 | |
| 1 × 102 | 34.13 ± 0.08 | 36.44 ± 0.04 | 31.88 ± 0.15 | |
| 10 | 37.29 ± 1.54 | 39.72 ± 0.23 | 33.16 ± 1.00 | |
| 1 | 38.52 ± 1.10 | 41.60 ± 0.42 | 32.41 ± 0.57 | |